BAIT

FKS1

CND1, CWH53, ETG1, GSC1, PBR1, 1,3-beta-D-glucan synthase, L000000445, L000000732, YLR342W

Catalytic subunit of 1,3-beta-D-glucan synthase; functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling; FKS1 has a paralog, GSC2, that arose from the whole genome duplication

GO Process (3)

GO Function (1)

GO Component (7)

Gene Ontology Biological Process

(1->3)-beta-D-glucan biosynthetic process [IDA, IMP]

positive regulation of endocytosis [IMP]

regulation of cell size [IMP]

Gene Ontology Molecular Function

1,3-beta-D-glucan synthase activity [IDA, IMP]

Gene Ontology Cellular Component

1,3-beta-D-glucan synthase complex [IDA]

actin cortical patch [IDA]

cellular bud neck [IDA]

cellular bud tip [IDA]

integral component of membrane [ISM]

mitochondrion [IDA]

plasma membrane [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

EPO1

YMR124W, YMR124W

Protein involved in septin-ER tethering; interacts with ER membrane protein, Scs2p, and Shs1p, a septin ring component, at bud neck to create ER diffusion barrier; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W has a paralog, YLR031W, that arose from the whole genome duplication

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Investigation of RNA metabolism through large-scale genetic interaction profiling in yeast.

Decourty L, Malabat C, Frachon E, Jacquier A, Saveanu C

Gene deletion and gene expression alteration can lead to growth defects that are amplified or reduced when a second mutation is present in the same cells. We performed 154 genetic interaction mapping (GIM) screens with query mutants related with RNA metabolism and estimated the growth rates of about 700 000 double mutant Saccharomyces cerevisiae strains. The tested targets included the gene ... [more]

Nucleic Acids Res Aug. 06, 2021; (); [Pubmed: 34358317]

Throughput

High Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)

Additional Notes

479 genetic interactions (GIs) were detected by both genetic interaction mapping (GIM) and SGA screens (adjusted log2(Q/R) score lower than -1 and SGA fitness score lower than -0.1).
Supplementary table S6

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FKS1 EPO1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.16	BioGRID	400707
FKS1 EPO1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2886	BioGRID	2154633

Curated By

BioGRID

Interaction Summary