BAIT

NFKB2

CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p100, p52
nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Multi-Omics Profiling to Assess Signaling Changes upon VHL Restoration and Identify Putative VHL Substrates in Clear Cell Renal Cell Carcinoma Cell Lines.

Wang X, Hu J, Fang Y, Fu Y, Liu B, Zhang C, Feng S, Lu X

The inactivation of von Hippel-Lindau (VHL) is critical for clear cell renal cell carcinoma (ccRCC) and VHL syndrome. VHL loss leads to the stabilization of hypoxia-inducible factor ? (HIF?) and other substrate proteins, which, together, drive various tumor-promoting pathways. There is inadequate molecular characterization of VHL restoration in VHL-defective ccRCC cells. The identities of HIF-independent VHL substrates remain elusive. We ... [more]

Cells Dec. 29, 2021; 11(3); [Pubmed: 35159281]

Throughput

  • Low Throughput

Curated By

  • BioGRID