BAIT

DTX2

RNF58

deltex 2, E3 ubiquitin ligase

GO Process (0)

GO Function (1)

GO Component (2)

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

nuclear membrane [IDA]

nucleoplasm [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

PREY

DAG1

156DAG, A3a, AGRNR, DAG, MDDGC7, MDDGC9

dystroglycan 1 (dystrophin-associated glycoprotein 1)

GO Process (9)

GO Function (8)

GO Component (13)

Gene Ontology Biological Process

NLS-bearing protein import into nucleus [IDA]

cytoskeletal anchoring at plasma membrane [IMP]

extracellular matrix organization [TAS]

membrane protein ectodomain proteolysis [IDA]

microtubule anchoring [IMP]

modulation by virus of host morphology or physiology [IDA]

negative regulation of MAPK cascade [IMP]

negative regulation of cell migration [IMP]

negative regulation of protein kinase B signaling [IMP]

Gene Ontology Molecular Function

SH2 domain binding [IDA]
actin binding [IDA]
alpha-actinin binding [IDA]
laminin-1 binding [ISS]
protein binding [IPI]
structural constituent of muscle [IMP]
tubulin binding [IDA]
vinculin binding [IPI]

Gene Ontology Cellular Component

basement membrane [IDA]

contractile ring [IDA]

cytoplasm [IDA]

dystrophin-associated glycoprotein complex [IDA]

extracellular region [TAS]

extracellular space [IDA]

extracellular vesicular exosome [IDA]

filopodium [IDA]

focal adhesion [IDA]

integral component of membrane [IDA]

lamellipodium [IDA]

nucleoplasm [IDA]

plasma membrane [IDA, TAS]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

An inducible CRISPR/Cas9 screen identifies DTX2 as a transcriptional regulator of human telomerase.

Zhou Z, Li Y, Xu H, Xie X, He Z, Lin S, Li R, Jin S, Cui J, Hu H, Liu F, Wu S, Ma W, Songyang Z

Most tumor cells reactivate telomerase to ensure unlimited proliferation, whereas the expression of human telomerase reverse transcriptase (hTERT) is tightly regulated and rate-limiting for telomerase activity maintenance. Several general transcription factors (TFs) have been found in regulating hTERT transcription; however, a systematic study is lacking. Here we performed an inducible CRISPR/Cas9 KO screen using an hTERT core promoter-driven reporter. We ... [more]

iScience Feb. 18, 2022; 25(2);103813 [Pubmed: 35198878]

Throughput

Low Throughput

Additional Notes

BioID

Curated By

BioGRID