BAIT

DTX2

RNF58

deltex 2, E3 ubiquitin ligase

GO Process (0)

GO Function (1)

GO Component (2)

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

nuclear membrane [IDA]

nucleoplasm [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

VAPB

ALS8, VAMP-B, VAP-B, RP5-1018E9.1

VAMP (vesicle-associated membrane protein)-associated protein B and C

GO Process (16)

GO Function (7)

GO Component (5)

Gene Ontology Biological Process

COPII-coated vesicle budding [IMP]

ER to Golgi vesicle-mediated transport [IMP]

activation of signaling protein activity involved in unfolded protein response [IDA]

cellular calcium ion homeostasis [IMP]

endoplasmic reticulum organization [IMP]

endoplasmic reticulum unfolded protein response [IMP]

modulation by virus of host morphology or physiology [IDA]

negative regulation by host of viral genome replication [IDA]

negative regulation by host of viral release from host cell [IDA]

negative regulation by virus of viral protein levels in host cell [IDA]

positive regulation by host of viral genome replication [IDA]

positive regulation by host of viral release from host cell [IDA]

positive regulation of viral genome replication [IMP]

small molecule metabolic process [TAS]

sphingolipid biosynthetic process [TAS]

sphingolipid metabolic process [TAS]

Gene Ontology Molecular Function

FFAT motif binding [IMP]
beta-tubulin binding [IDA]
enzyme binding [IPI]
microtubule binding [IDA]
protein binding [IPI]
protein heterodimerization activity [IPI]
protein homodimerization activity [IPI]

Gene Ontology Cellular Component

Golgi apparatus [IDA]

Golgi membrane [IMP]

endoplasmic reticulum [IDA]

endoplasmic reticulum exit site [IDA]

endoplasmic reticulum membrane [IDA, TAS]

CRISPR Database VEGA HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

An inducible CRISPR/Cas9 screen identifies DTX2 as a transcriptional regulator of human telomerase.

Zhou Z, Li Y, Xu H, Xie X, He Z, Lin S, Li R, Jin S, Cui J, Hu H, Liu F, Wu S, Ma W, Songyang Z

Most tumor cells reactivate telomerase to ensure unlimited proliferation, whereas the expression of human telomerase reverse transcriptase (hTERT) is tightly regulated and rate-limiting for telomerase activity maintenance. Several general transcription factors (TFs) have been found in regulating hTERT transcription; however, a systematic study is lacking. Here we performed an inducible CRISPR/Cas9 KO screen using an hTERT core promoter-driven reporter. We ... [more]

iScience Feb. 18, 2022; 25(2);103813 [Pubmed: 35198878]

Throughput

Low Throughput

Additional Notes

BioID

Curated By

BioGRID