Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Neddylation modification of the U3 snoRNA-binding protein RRP9 by Smurf1 promotes tumorigenesis.

Du MG, Liu F, Chang Y, Tong S, Liu W, Chen YJ, Xie P

Neddylation is a posttranslational modification that attaches ubiquitin-like protein Nedd8 to protein targets via Nedd8-specific E1-E2-E3 enzymes and modulates many important biological processes. Nedd8 attaches to a lysine residue of a substrate, not for degradation, but for modulation of substrate activity. We previously identified the HECT-type ubiquitin ligase Smurf1, which controls diverse cellular processes, is activated by Nedd8 through covalent ... [more]

J Biol Chem Dec. 01, 2020; 297(5);101307 [Pubmed: 34662580]

Quantitative Score

  • 48.49 [Peptide ion score]

Throughput

  • High Throughput

Additional Notes

  • Affinity Capture-MS was carried out to identify high confidence protein-protein interactors with a Peptide ion score of greater than or equal to 20

Curated By

  • BioGRID