Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interactome Analysis of Human Phospholipase D and Phosphatidic Acid-Associated Protein Network.

Kattan RE, Han H, Seo G, Yang B, Lin Y, Dotson M, Pham S, Menely Y, Wang W

Mammalian phospholipase D (PLD) enzyme family consists of six members. Among them, PLD1/2/6 catalyzes phosphatidic acid (PA) production, while PLD3/4/5 has no catalytic activities. Deregulation of the PLD-PA lipid signaling has been associated with various human diseases including cancer. However, a comprehensive analysis of the regulators and effectors for this crucial lipid metabolic pathway has not been fully achieved. Using ... [more]

Mol Cell Proteomics Dec. 01, 2021; 21(2);100195 [Pubmed: 35007762]

Quantitative Score

  • 1.0 [Saint Score]

Throughput

  • High Throughput

Additional Notes

  • Affinity capture MS was carried out to identify high confidence protein interactors with a SAINT score > 0.8 and raw spectra counts >2 (SAINT score reported here)

Curated By

  • BioGRID