An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

EZH2 depletion potentiates MYC degradation inhibiting neuroblastoma and small cell carcinoma tumor formation.

Wang L, Chen C, Song Z, Wang H, Ye M, Wang D, Kang W, Liu H, Qing G

Efforts to therapeutically target EZH2 have generally focused on inhibition of its methyltransferase activity, although it remains less clear whether this is the central mechanism whereby EZH2 promotes cancer. In the current study, we show that EZH2 directly interacts with both MYC family oncoproteins, MYC and MYCN, and promotes their stabilization in a methyltransferase-independent manner. By competing against the SCFFBW7 ... [more]

Nat Commun Dec. 10, 2021; 13(1);12 [Pubmed: 35013218]

Throughput

High Throughput

Additional Notes

Affinity Capture-MS was carried out to identify high confidence protein interactors with a p value<0.05 and a fold change >2 over control.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PRNP MYCN	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Li H (2022)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MYCN

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]protein binding [IPI]

Gene Ontology Cellular Component

PRNP

Gene Ontology Biological Process

Gene Ontology Molecular Function

copper ion binding [IDA, TAS]identical protein binding [IPI]microtubule binding [IDA]protein binding [IPI]tubulin binding [IDA]