BAIT

C9ORF78

HCA59, HSPC220, bA409K20.3
chromosome 9 open reading frame 78
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens
PREY

CLTC

CHC, CHC17, CLH-17, CLTCL2, Hc
clathrin, heavy chain (Hc)
Autophagy Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A multi-factor trafficking site on the spliceosome remodeling enzyme BRR2 recruits C9ORF78 to regulate alternative splicing.

Bergfort A, Preussner M, Kuropka B, Ilik IA, Hilal T, Weber G, Freund C, Akta? T, Heyd F, Wahl MC

The intrinsically unstructured C9ORF78 protein was detected in spliceosomes but its role in splicing is presently unclear. We find that C9ORF78 tightly interacts with the spliceosome remodeling factor, BRR2, in vitro. Affinity purification/mass spectrometry and RNA UV-crosslinking analyses identify additional C9ORF78 interactors in spliceosomes. Cryogenic electron microscopy structures reveal how C9ORF78 and the spliceosomal B complex protein, FBP21, wrap around ... [more]

Nat Commun Dec. 03, 2021; 13(1);1132 [Pubmed: 35241646]

Throughput

  • High Throughput

Curated By

  • BioGRID