Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Loss of RPS27a expression regulates the cell cycle, apoptosis, and proliferation via the RPL11-MDM2-p53 pathway in lung adenocarcinoma cells.

Li H, Zhang H, Huang G, Bing Z, Xu D, Liu J, Luo H, An X

Depletion of certain ribosomal proteins induces p53 activation, which is mediated mainly by ribosomal protein L5 (RPL5) and/or ribosomal protein L11 (RPL11). Therefore, RPL5 and RPL11 may link RPs and p53 activation. Thus, this study aimed to explore whether RPs interact with RPL11 and regulate p53 activation in lung adenocarcinoma (LUAD) cells.The endogenous RPL11-binding proteins in A549 cells were pulled ... [more]

J Exp Clin Cancer Res Jan. 24, 2022; 41(1);33 [Pubmed: 35073964]

Throughput

  • Low Throughput

Curated By

  • BioGRID