Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Targeting USP9X-AMPK Axis in ARID1A-Deficient Hepatocellular Carcinoma.

Zhang FK, Ni QZ, Wang K, Cao HJ, Guan DX, Zhang EB, Ma N, Wang YK, Zheng QW, Xu S, Zhu B, Chen TW, Xia J, Qiu XS, Ding XF, Jiang H, Qiu L, Wang X, Chen W, Cheng SQ, Xie D, Li JJ

Hepatocellular carcinoma (HCC) is a highly heterogeneous solid tumor with high morbidity and mortality. AT-rich interaction domain 1A (ARID1A) accounts for up to 10% of mutations in liver cancer, however, its role in HCC remains controversial, and no targeted therapy has been established.The expression of ARID1A in clinical samples was examined by Western blot and immunohistochemical staining. ARID1A was knocked ... [more]

Cell Mol Gastroenterol Hepatol Apr. 08, 2022; 14(1);101-127 [Pubmed: 35390516]

Throughput

  • Low Throughput

Curated By

  • BioGRID