PREY

SPI

CG10334, CT29014, Dmel\CG10334, EP(2)2378, Spitz, anon-WO0118547.158, l(2)01068, l(2)37Fa, l(2)s3547, s-spi, sSpi, Dmel_CG10334
spitz
GO Process (25)
GO Function (2)
GO Component (5)
Drosophila melanogaster

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Lipid-mediated phase separation of AGO proteins on the ER controls nascent-peptide ubiquitination.

Gao Y, Zhu Y, Wang H, Cheng Y, Zhao D, Sun Q, Chen D

AGO/miRNA-mediated gene silencing and ubiquitin-mediated protein quality control represent two fundamental mechanisms that control proper gene expression. Here, we unexpectedly discover that fly and human AGO proteins, which are key components in the miRNA pathway, undergo lipid-mediated phase separation and condense into RNP granules on the endoplasmic reticulum (ER) membrane to control protein production. Phase separation on the ER is ... [more]

Mol Cell Dec. 07, 2021; 82(7);1313-1328.e8 [Pubmed: 35325613]

Throughput

  • High Throughput

Curated By

  • BioGRID