Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

c-MYC-USP49-BAG2 axis promotes proliferation and chemoresistance of colorectal cancer cells in vitro.

Tu R, Kang W, Kang Y, Chen Z, Zhang P, Xiong X, Ma J, Du RL, Zhang C

Deubiquitinases (DUBs) play critical roles in tumorigenesis and are emerging as potential therapeutic targets. However, it remains less clear which DUBs may play important roles and represent a realistic vulnerability for a particular type of tumor. Here we revealed that Ubiquitin Specific Peptidase 49 (USP49) is transcriptionally activated by c-MYC in colorectal cancer (CRC), and CRC patients with elevated USP49 ... [more]

Biochem Biophys Res Commun Dec. 04, 2021; 607();117-123 [Pubmed: 35367823]

Throughput

  • High Throughput

Curated By

  • BioGRID