PREY

UBE2C

UBCH10, dJ447F3.2, RP3-447F3.1
ubiquitin-conjugating enzyme E2C
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Regulation of p27 (Kip1) by Ubiquitin E3 Ligase RNF6.

Deshmukh D, Xu J, Yang X, Shimelis H, Fang S, Qiu Y

The cyclin-dependent kinase inhibitor p27 (Kip1) is an important regulator of the G1/S checkpoint. It is degraded by the SCF-SKP2 complex in late G1 thereby allowing cells to progress to the S phase. Here we investigated the role of the E3 ubiquitin ligase RNF6 (Ring Finger Protein 6) in cell cycle progression in prostate cancer cells. Our data demonstrate that ... [more]

Pharmaceutics Apr. 06, 2022; 14(4); [Pubmed: 35456636]

Throughput

  • Low Throughput

Curated By

  • BioGRID