BAIT
PARP1
ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1, RP11-125A15.2
poly (ADP-ribose) polymerase 1
GO Process (12)
GO Function (7)
GO Component (6)
Gene Ontology Biological Process
- DNA repair [TAS]
- cellular response to insulin stimulus [IDA]
- double-strand break repair [IMP]
- gene expression [TAS]
- macrophage differentiation [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- protein ADP-ribosylation [IDA]
- protein poly-ADP-ribosylation [IDA]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CLIC4
CLIC4L, H1, MTCLIC, huH1, p64H1
chloride intracellular channel 4
GO Process (8)
GO Function (2)
GO Component (16)
Gene Ontology Biological Process
- cell differentiation [TAS]
- cellular response to calcium ion [IMP]
- chloride transmembrane transport [TAS]
- chloride transport [NAS]
- establishment or maintenance of apical/basal cell polarity [NAS]
- keratinocyte differentiation [IMP]
- negative regulation of cell migration [IDA]
- regulation of cytoskeleton organization [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin cytoskeleton [TAS]
- apical part of cell [IDA]
- cell surface [IDA]
- cell-cell junction [IDA]
- centrosome [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- extracellular vesicular exosome [IDA]
- intracellular [IDA]
- microtubule cytoskeleton [IDA]
- microvillus [IDA]
- midbody [IDA]
- mitochondrion [IDA]
- nuclear matrix [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin.
Poly (ADP-ribose) polymerase (PARP) inhibitors elicit antitumour activity in homologous recombination-defective cancers by trapping PARP1 in a chromatin-bound state. How cells process trapped PARP1 remains unclear. Using wild-type and a trapping-deficient PARP1 mutant combined with rapid immunoprecipitation mass spectrometry of endogenous proteins and Apex2 proximity labelling, we delineated mass spectrometry-based interactomes of trapped and non-trapped PARP1. These analyses identified an ... [more]
Nat Cell Biol Dec. 01, 2021; 24(1);62-73 [Pubmed: 35013556]
Throughput
- High Throughput
Additional Notes
- Proximity Label-MS was carried out to identify high confidence protein-protein interactors.
Curated By
- BioGRID