An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin.

Krastev DB, Li S, Sun Y, Wicks AJ, Hoslett G, Weekes D, Badder LM, Knight EG, Marlow R, Pardo MC, Yu L, Talele TT, Bartek J, Choudhary JS, Pommier Y, Pettitt SJ, Tutt ANJ, Ramadan K, Lord CJ

Poly (ADP-ribose) polymerase (PARP) inhibitors elicit antitumour activity in homologous recombination-defective cancers by trapping PARP1 in a chromatin-bound state. How cells process trapped PARP1 remains unclear. Using wild-type and a trapping-deficient PARP1 mutant combined with rapid immunoprecipitation mass spectrometry of endogenous proteins and Apex2 proximity labelling, we delineated mass spectrometry-based interactomes of trapped and non-trapped PARP1. These analyses identified an ... [more]

Nat Cell Biol Dec. 01, 2021; 24(1);62-73 [Pubmed: 35013556]

Throughput

High Throughput

Additional Notes

Proximity Label-MS was carried out to identify high confidence protein-protein interactors.

Curated By

BioGRID

Interaction Summary

PARP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NAD+ ADP-ribosyltransferase activity [IDA, TAS]enzyme binding [IPI]identical protein binding [IPI]poly(A) RNA binding [IDA]protein N-terminus binding [IPI]protein binding [IPI]transcription factor binding [IPI]

Gene Ontology Cellular Component

CPT2