Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

CD95/Fas suppresses NF-?B activation through recruitment of KPC2 in a CD95L/FasL-independent mechanism.

Guegan JP, Pollet J, Ginestier C, Charafe-Jauffret E, Peter ME, Legembre P

CD95 expression is preserved in triple-negative breast cancers (TNBCs), and CD95 loss in these cells triggers the induction of a pro-inflammatory program, promoting the recruitment of cytotoxic NK cells impairing tumor growth. Herein, we identify a novel interaction partner of CD95, Kip1 ubiquitination-promoting complex protein 2 (KPC2), using an unbiased proteomic approach. Independently of CD95L, CD95/KPC2 interaction contributes to the ... [more]

iScience Dec. 17, 2021; 24(12);103538 [Pubmed: 34917906]

Throughput

  • High Throughput

Additional Notes

  • Proximity Label-MS was carried out to identify high confidence protein-protein interactors.

Curated By

  • BioGRID