Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Protective function of the SQSTM1/p62-NEDD4 complex against methylmercury toxicity.

Takanezawa Y, Harada R, Shibagaki Y, Kashiwano Y, Nakamura R, Ohshiro Y, Uraguchi S, Kiyono M

SQSTM1/p62, hereinafter referred to as p62, is a stress-induced cellular protein that interacts with various signaling proteins as well as ubiquitinated proteins to regulate a variety of cellular functions and cell survival. Methylmercury (MeHg) exposure increases the levels of p62, the latter playing a protective role in MeHg-induced toxicity. However, the underlying mechanism by which p62 alleviates MeHg toxicity remains ... [more]

Biochem Biophys Res Commun Dec. 18, 2021; 609();134-140 [Pubmed: 35452957]

Throughput

  • Low Throughput

Curated By

  • BioGRID