BAIT

GAT1

MEP80, NIL1, L000002835, YFL021W

Transcriptional activator of nitrogen catabolite repression genes; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p; different translational starts produce two major and two minor isoforms that are differentially regulated and localized

GO Process (2)

GO Function (3)

GO Component (2)

Gene Ontology Biological Process

nitrogen catabolite activation of transcription [IGI, IMP]

nitrogen catabolite activation of transcription from RNA polymerase II promoter [IGI, IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
RNA polymerase II transcription factor binding transcription factor activity [IMP]
sequence-specific DNA binding [IDA, IGI]

Gene Ontology Cellular Component

cytosol [IDA]

nucleus [IDA, IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

CAT8

DIL1, MSP8, DNA-binding transcription factor CAT8, L000000220, YMR280C

Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress

GO Process (3)

GO Function (3)

GO Component (2)

Gene Ontology Biological Process

positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter [IMP]

positive regulation of glyoxylate cycle by positive regulation of transcription from RNA polymerase II promoter [IMP]

positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleus [IC, IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cat8 Response to Nutritional Changes and Interaction With Ehrlich Pathway Related Factors.

Du Z, Deng H, Cheng Y, Zhai Z, Guo X, Wang Z, He X

Cat8 is an important transcription factor regulating the utilization of non-fermentative carbon sources in Saccharomyces cerevisiae. However, our previous studies found that Cat8 may play a critical role in nitrogen metabolism, but the regulatory mechanism has not been elucidated. In this study, the nuclear localization and analysis of regulatory activity showed that the Cat8 function relies on Snf1 kinase. In ... [more]

Front Microbiol Jul. 06, 2022; 13();898938 [Pubmed: 35783377]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CAT8 GAT1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Du Z (2022)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GAT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]RNA polymerase II transcription factor binding transcription factor activity [IMP]sequence-specific DNA binding [IDA, IGI]

Gene Ontology Cellular Component

CAT8

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-Western

Publication

Cat8 Response to Nutritional Changes and Interaction With Ehrlich Pathway Related Factors.

Throughput

Related interactions

Curated By

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
RNA polymerase II transcription factor binding transcription factor activity [IMP]
sequence-specific DNA binding [IDA, IGI]

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
sequence-specific DNA binding [IDA]