RFFL
Gene Ontology Biological Process
- negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis [IMP]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [IMP]
- negative regulation of signal transduction by p53 class mediator [IMP]
- negative regulation of tumor necrosis factor-mediated signaling pathway [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein K48-linked ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- regulation of TOR signaling [IC]
- regulation of fibroblast migration [ISS]
- ubiquitin-dependent protein catabolic process [IDA, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UQCRC1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Endosomal-associated RFFL facilitates mitochondrial clearance by enhancing PRKN/parkin recruitment to mitochondria.
Mutations in the ubiquitin ligase PRKN (parkin RBR E3 ubiquitin protein ligase) are associated with Parkinson disease and defective mitophagy. Conceptually, PRKN-dependent mitophagy is classified into two phases: 1. PRKN recruits to and ubiquitinates mitochondrial proteins; 2. formation of phagophore membrane, sequestering mitochondria for degradation. Recently, endosomal machineries are reported to contribute to the later stage for membrane assembly. We ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID