BAIT
TMEM173
2610307O08Rik, ERIS, MPYS, Mita, STING
transmembrane protein 173
GO Process (11)
GO Function (8)
GO Component (6)
Gene Ontology Biological Process
- activation of innate immune response [IMP, ISO]
- cellular response to exogenous dsRNA [ISO]
- cellular response to interferon-beta [IMP]
- defense response to virus [IMP, ISO]
- innate immune response [IMP, ISO]
- interferon-beta production [IMP, ISO]
- positive regulation of defense response to virus by host [ISO]
- positive regulation of protein binding [ISO]
- positive regulation of protein import into nucleus, translocation [ISO]
- positive regulation of transcription factor import into nucleus [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
VAMP3
D130027G05Rik, VAMP-3, ceb, RP23-455G15.3
vesicle-associated membrane protein 3
GO Process (11)
GO Function (4)
GO Component (15)
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [IMP]
- SNARE complex assembly [IDA]
- calcium ion-dependent exocytosis [IMP]
- negative regulation of secretion by cell [ISO]
- neurotransmitter secretion [IBA]
- positive regulation of receptor recycling [IDA]
- protein complex assembly [ISO]
- retrograde transport, endosome to Golgi [ISO]
- substrate adhesion-dependent cell spreading [IDA]
- vesicle fusion [IBA]
- vesicle-mediated transport [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IBA, ISO]
- apical plasma membrane [ISO]
- cell [IMP]
- cell surface [IDA]
- clathrin-coated vesicle [ISO]
- clathrin-coated vesicle membrane [ISO]
- cytoplasmic vesicle [IDA]
- cytosol [IDA, ISO]
- intracellular [IMP, ISO]
- intracellular membrane-bounded organelle [ISO]
- intracellular organelle [ISO]
- plasma membrane [IDA, ISO]
- recycling endosome [ISO]
- secretory granule [IDA]
- synaptic vesicle [IBA]
Mus musculus
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
BioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING).
Stimulator of interferon genes (STING) mediates cytosolic DNA-induced innate immune signaling via membrane trafficking. The global identification of proteins that spatiotemporally interact with STING will provide a better understanding of its trafficking mechanisms and of STING signaling pathways. Proximity-dependent biotin identification (BioID) is a powerful technology to identify physiologically relevant protein-protein interactions in living cells. However, biotinylated peptides are rarely ... [more]
J Biol Chem Dec. 07, 2019; 295(32);11174-11183 [Pubmed: 32554809]
Throughput
- High Throughput
Additional Notes
- TurboID fused-STING, peptides were filtered at a false discovery rate (FDR) of 1%.
Curated By
- BioGRID