An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

BioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING).

Motani K, Kosako H

Stimulator of interferon genes (STING) mediates cytosolic DNA-induced innate immune signaling via membrane trafficking. The global identification of proteins that spatiotemporally interact with STING will provide a better understanding of its trafficking mechanisms and of STING signaling pathways. Proximity-dependent biotin identification (BioID) is a powerful technology to identify physiologically relevant protein-protein interactions in living cells. However, biotinylated peptides are rarely ... [more]

J Biol Chem Dec. 07, 2019; 295(32);11174-11183 [Pubmed: 32554809]

Throughput

High Throughput

Additional Notes

TurboID fused-STING, peptides were filtered at a false discovery rate (FDR) of 1%.

Curated By

BioGRID

Interaction Summary

TMEM173

Gene Ontology Biological Process

Gene Ontology Molecular Function

cyclic-GMP-AMP binding [IDA]cyclic-di-GMP binding [IDA, ISO]identical protein binding [ISO]protein binding [IPI]protein homodimerization activity [ISO]protein kinase binding [ISO]transcription factor binding [ISO]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

MBP