BAIT
GOLPH3L
GPP34R, RP11-54A4.7
golgi phosphoprotein 3-like
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ARCN1
COPD
archain 1
GO Process (3)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
GOLPH3 and GOLPH3L are broad-spectrum COPI adaptors for sorting into intra-Golgi transport vesicles.
The fidelity of Golgi glycosylation is, in part, ensured by compartmentalization of enzymes within the stack. The COPI adaptor GOLPH3 has been shown to interact with the cytoplasmic tails of a subset of Golgi enzymes and direct their retention. However, other mechanisms of retention, and other roles for GOLPH3, have been proposed, and a comprehensive characterization of the clientele of ... [more]
J Cell Biol Dec. 04, 2020; 220(10); [Pubmed: 34473204]
Throughput
- High Throughput
Additional Notes
- Used whole cell lysate GST-tagged GOLPH3 compared to a GST only control
- Confirmed by MS
Curated By
- BioGRID