Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The ATG5 interactome links clathrin-mediated vesicular trafficking with the autophagosome assembly machinery.

Baines K, Yoshioka K, Takuwa Y, Lane JD

Autophagosome formation involves the sequential actions of conserved ATG proteins to coordinate the lipidation of the ubiquitin-like modifier Atg8-family proteins at the nascent phagophore membrane. Although the molecular steps driving this process are well understood, the source of membranes for the expanding phagophore and their mode of delivery are only now beginning to be revealed. Here, we have used quantitative ... [more]

Autophagy Rep Apr. 23, 2022; 1(1);88-118 [Pubmed: 35449600]

Throughput

  • Low Throughput

Curated By

  • BioGRID