BAIT

RPT5

YTA1, proteasome regulatory particle base subunit RPT5, L000002555, YOR117W
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

RPS27B

ribosomal 40S subunit protein S27B, S27e, rp61, YS20, S27B, L000002713, YHR021C
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Characterization of the proteasome interaction network using a QTAX-based tag-team strategy and protein interaction network analysis.

Guerrero C, Milenkovic T, Przulj N, Kaiser P, Huang L

Quantitative analysis of tandem-affinity purified cross-linked (x) protein complexes (QTAX) is a powerful technique for the identification of protein interactions, including weak and/or transient components. Here, we apply a QTAX-based tag-team mass spectrometry strategy coupled with protein network analysis to acquire a comprehensive and detailed assessment of the protein interaction network of the yeast 26S proteasome. We have determined that ... [more]

Proc. Natl. Acad. Sci. U.S.A. Sep. 09, 2008; 105(36);13333-8 [Pubmed: 18757749]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPT5 RPS27B
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1297BioGRID
2016149

Curated By

  • BioGRID