BAIT
TP53BP1
53BP1, p202
tumor protein p53 binding protein 1
GO Process (8)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
- DNA repair [TAS]
- cellular response to DNA damage stimulus [IDA]
- double-strand break repair [TAS]
- double-strand break repair via homologous recombination [TAS]
- positive regulation of sequence-specific DNA binding transcription factor activity [IC]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription, DNA-templated [NAS]
- transcription from RNA polymerase II promoter [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
APEX1
APE, APE1, APEN, APEX, APX, HAP1, REF1
APEX nuclease (multifunctional DNA repair enzyme) 1
GO Process (9)
GO Function (19)
GO Component (10)
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [IDA, TAS]
- DNA catabolic process, exonucleolytic [IBA]
- DNA demethylation [IDA]
- DNA repair [IDA, TAS]
- base-excision repair [IBA, TAS]
- negative regulation of nucleic acid-templated transcription [TAS]
- oxidation-reduction process [IDA]
- positive regulation of DNA repair [IDA]
- regulation of mRNA stability [IMP]
Gene Ontology Molecular Function- 3'-5' exonuclease activity [IDA, TAS]
- DNA binding [IDA]
- DNA-(apurinic or apyrimidinic site) lyase activity [IDA, TAS]
- RNA-DNA hybrid ribonuclease activity [TAS]
- chromatin DNA binding [IDA]
- damaged DNA binding [IDA]
- double-stranded DNA 3'-5' exodeoxyribonuclease activity [IBA]
- endodeoxyribonuclease activity [TAS]
- endonuclease activity [IDA]
- metal ion binding [IDA]
- oxidoreductase activity [IDA]
- phosphodiesterase I activity [TAS]
- phosphoric diester hydrolase activity [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- site-specific endodeoxyribonuclease activity, specific for altered base [IDA]
- transcription coactivator activity [IDA]
- transcription corepressor activity [TAS]
- uracil DNA N-glycosylase activity [TAS]
- 3'-5' exonuclease activity [IDA, TAS]
- DNA binding [IDA]
- DNA-(apurinic or apyrimidinic site) lyase activity [IDA, TAS]
- RNA-DNA hybrid ribonuclease activity [TAS]
- chromatin DNA binding [IDA]
- damaged DNA binding [IDA]
- double-stranded DNA 3'-5' exodeoxyribonuclease activity [IBA]
- endodeoxyribonuclease activity [TAS]
- endonuclease activity [IDA]
- metal ion binding [IDA]
- oxidoreductase activity [IDA]
- phosphodiesterase I activity [TAS]
- phosphoric diester hydrolase activity [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- site-specific endodeoxyribonuclease activity, specific for altered base [IDA]
- transcription coactivator activity [IDA]
- transcription corepressor activity [TAS]
- uracil DNA N-glycosylase activity [TAS]
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Genome-wide CRISPR screens using isogenic cells reveal vulnerabilities conferred by loss of tumor suppressors.
Exploiting cancer vulnerabilities is critical for the discovery of anticancer drugs. However, tumor suppressors cannot be directly targeted because of their loss of function. To uncover specific vulnerabilities for cells with deficiency in any given tumor suppressor(s), we performed genome-scale CRISPR loss-of-function screens using a panel of isogenic knockout cells we generated for 12 common tumor suppressors. Here, we provide ... [more]
Sci Adv Dec. 13, 2021; 8(19);eabm6638 [Pubmed: 35559673]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507) [viability (PATO:0000169)]
Additional Notes
- CRISPR GI screen
- Cell Line:HEK-293A
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: TKO v3 (ADDGENE:90294)
- Significance Threshold: BAGEL (QBF_X_TSG KO>5, QBF_X_WT_Average<3, and QBF_X_TSG KO - QBF_X_WT_Max>5, where X is any gene from TKOv3 library) and DrugZ (P<0.01)
- Significance Threshold: BAGEL (QBF_X_TSG KO>5, QBF_X_WT_Average<3, and QBF_X_TSG KO - QBF_X_WT_Max>5, where X is any gene from TKOv3 library) and DrugZ (P<0.01)
Curated By
- BioGRID