BAIT

RPS3

SUF14, ribosomal 40S subunit protein S3, rp13, YS3, S3, L000002161, L000001749, YNL178W
Protein component of the small (40S) ribosomal subunit; has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
Saccharomyces cerevisiae (S288c)
PREY

PMA2

H(+)-exporting P2-type ATPase PMA2, L000001450, YPL036W
Plasma membrane H+-ATPase; isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A Multi-Perspective Proximity View on the Dynamic Head Region of the Ribosomal 40S Subunit.

Schmitt K, Kraft AA, Valerius O

A comparison of overlapping proximity captures at the head region of the ribosomal 40S subunit (hr40S) in Saccharomyces cerevisiae from four adjacent perspectives, namely Asc1/RACK1, Rps2/uS5, Rps3/uS3, and Rps20/uS10, corroborates dynamic co-localization of proteins that control activity and fate of both ribosomes and mRNA. Co-locating factors that associate with the hr40S are involved in (i) (de)ubiquitination of ribosomal proteins (Hel2, ... [more]

Int J Mol Sci Oct. 28, 2021; 22(21); [Pubmed: 34769086]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • greater or equal to 1.5-fold enrichment, p-value threshold of 0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPS3 PMA2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2224BioGRID
2010306

Curated By

  • BioGRID