BAIT
CNOT8
CAF1, CALIF, Caf1b, POP2, hCAF1
CCR4-NOT transcription complex, subunit 8
GO Process (11)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- RNA phosphodiester bond hydrolysis, exonucleolytic [IDA]
- exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay [IDA]
- gene expression [TAS]
- gene silencing by miRNA [TAS]
- mRNA metabolic process [TAS]
- negative regulation of cell proliferation [TAS]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA poly(A) tail shortening [TAS]
- positive regulation of cell proliferation [IMP]
- regulation of transcription, DNA-templated [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CNOT7
CAF1, Caf1a, hCAF-1
CCR4-NOT transcription complex, subunit 7
GO Process (15)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- RNA phosphodiester bond hydrolysis, exonucleolytic [IDA]
- carbohydrate metabolic process [TAS]
- exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay [IDA]
- gene expression [TAS]
- gene silencing by RNA [ISS]
- gene silencing by miRNA [TAS]
- mRNA metabolic process [TAS]
- negative regulation of cell proliferation [IDA]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA poly(A) tail shortening [TAS]
- positive regulation of cell proliferation [IMP]
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IMP]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [IMP]
- signal transduction [TAS]
Gene Ontology Molecular Function
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Combinatorial CRISPR screen identifies fitness effects of gene paralogues.
Genetic redundancy has evolved as a way for human cells to survive the loss of genes that are single copy and essential in other organisms, but also allows tumours to survive despite having highly rearranged genomes. In this study we CRISPR screen 1191 gene pairs, including paralogues and known and predicted synthetic lethal interactions to identify 105 gene combinations whose ... [more]
Nat Commun Dec. 26, 2020; 12(1);1302 [Pubmed: 33637726]
Throughput
- Low Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507) [viability (PATO:0000169)]
- growth abnormality (HP:0001507) [viability (PATO:0000169)]
Additional Notes
- CRISPR GI screen
- Cell Line: A-375 cell BTO:0002806
- Cell Line: MEWO cell BTO:0003301
- Cell Line: RPE-1
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Targeted CRISPR synthetic lethality library
- Significance Threshold:FDR<0.1 and additional filtering of gene pairs with one gene with a large individual fitness defect
Curated By
- BioGRID