Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Combinatorial CRISPR screen identifies fitness effects of gene paralogues.

Thompson NA, Ranzani M, van der Weyden L, Iyer V, Offord V, Droop A, Behan F, Goncalves E, Speak A, Iorio F, Hewinson J, Harle V, Robertson H, Anderson E, Fu B, Yang F, Zagnoli-Vieira G, Chapman P, Del Castillo Velasco-Herrera M, Garnett MJ, Jackson SP, Adams DJ

Genetic redundancy has evolved as a way for human cells to survive the loss of genes that are single copy and essential in other organisms, but also allows tumours to survive despite having highly rearranged genomes. In this study we CRISPR screen 1191 gene pairs, including paralogues and known and predicted synthetic lethal interactions to identify 105 gene combinations whose ... [more]

Nat Commun Dec. 26, 2020; 12(1);1302 [Pubmed: 33637726]

Throughput

Low Throughput

Ontology Terms

growth abnormality (HP:0001507) [viability (PATO:0000169)]

Additional Notes

CRISPR GI screen
Cell Line: RPE-1
Experimental Setup: Timecourse
GIST: A-phenotypic negative genetic interaction
Library: Targeted CRISPR synthetic lethality library
Significance Threshold:FDR<0.1 and additional filtering of gene pairs with one gene with a large individual fitness defect

Curated By

BioGRID

Interaction Summary

ALKBH4

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [IDA]demethylase activity [TAS]oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]

Gene Ontology Cellular Component

ATR

Gene Ontology Biological Process

Gene Ontology Molecular Function

MutLalpha complex binding [IDA]MutSalpha complex binding [IDA]protein binding [IPI]protein dimerization activity [IBA]protein kinase activity [IDA]protein serine/threonine kinase activity [IDA]