ARFGEF1
Gene Ontology Biological Process
- Golgi organization [IMP]
- endomembrane system organization [IMP]
- exocytosis [TAS]
- negative regulation of Rho GTPase activity [IDA]
- negative regulation of actin filament polymerization [IMP]
- positive regulation of GTPase activity [IBA, IDA]
- positive regulation of protein glycosylation in Golgi [IMP]
- positive regulation of wound healing [IMP]
- regulation of establishment of cell polarity [IMP]
- vesicle-mediated transport [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ARFGEF2
Gene Ontology Biological Process
- Golgi to plasma membrane transport [IMP]
- endomembrane system organization [IMP]
- endosome organization [IMP]
- intracellular signal transduction [IDA]
- positive regulation of GTPase activity [IBA, IDA]
- positive regulation of tumor necrosis factor production [IMP]
- receptor recycling [IDA]
- vesicle-mediated transport [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Combinatorial CRISPR screen identifies fitness effects of gene paralogues.
Genetic redundancy has evolved as a way for human cells to survive the loss of genes that are single copy and essential in other organisms, but also allows tumours to survive despite having highly rearranged genomes. In this study we CRISPR screen 1191 gene pairs, including paralogues and known and predicted synthetic lethal interactions to identify 105 gene combinations whose ... [more]
Throughput
- Low Throughput
Ontology Terms
- growth abnormality (HP:0001507) [viability (PATO:0000169)]
Additional Notes
- CRISPR GI screen
- Cell Line: RPE-1
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Targeted CRISPR synthetic lethality library
- Significance Threshold:FDR<0.1 and additional filtering of gene pairs with one gene with a large individual fitness defect
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ARFGEF1 ARFGEF2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3347934 | |
| ARFGEF2 ARFGEF1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3347940 | |
| ARFGEF1 ARFGEF2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ARFGEF2 ARFGEF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ARFGEF1 ARFGEF2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | - | |
| ARFGEF1 ARFGEF2 | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | - |
Curated By
- BioGRID