BAIT
ARF4
ARF2
ADP-ribosylation factor 4
GO Process (6)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
COL4A3
collagen, type IV, alpha 3 (Goodpasture antigen)
GO Process (14)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- axon guidance [TAS]
- blood circulation [TAS]
- cell proliferation [IDA]
- cell surface receptor signaling pathway [NAS]
- collagen catabolic process [TAS]
- endothelial cell apoptotic process [IDA]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- glomerular basement membrane development [ISS]
- negative regulation of angiogenesis [IDA]
- negative regulation of cell proliferation [TAS]
- negative regulation of endopeptidase activity [NAS]
- sensory perception of sound [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.
The Arf GTPase family is involved in a wide range of cellular regulation including membrane trafficking and organelle-structure assembly. Here, we have generated a proximity interaction network for the Arf family using the miniTurboID approach combined with TMT-based quantitative mass spectrometry. Our interactome confirmed known interactions and identified many novel interactors that provide leads for defining Arf pathway cell biological ... [more]
EMBO J Jul. 17, 2022; ();e110698 [Pubmed: 35844135]
Throughput
- High Throughput
Additional Notes
- Constitutively active mutant of GTPase fused with miniTurboID was stably expressed in HEK293A cells, followed by miniTurboID experiment and TMT-based mass spectrometry. FDR threshold of 0.05
Curated By
- BioGRID