BAIT
ARF5
ADP-ribosylation factor 5
GO Process (1)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CAMK4
CaMK IV, CaMK-GR, IV, caMK
calcium/calmodulin-dependent protein kinase IV
GO Process (13)
GO Function (0)
GO Component (4)
Gene Ontology Biological Process
- activation of phospholipase C activity [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- long-term memory [IGI]
- myeloid dendritic cell cytokine production [IMP]
- myeloid dendritic cell differentiation [IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of T cell differentiation in thymus [TAS]
- regulation of osteoclast differentiation [TAS]
- signal transduction [TAS]
- synaptic transmission [TAS]
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.
The Arf GTPase family is involved in a wide range of cellular regulation including membrane trafficking and organelle-structure assembly. Here, we have generated a proximity interaction network for the Arf family using the miniTurboID approach combined with TMT-based quantitative mass spectrometry. Our interactome confirmed known interactions and identified many novel interactors that provide leads for defining Arf pathway cell biological ... [more]
EMBO J Jul. 17, 2022; ();e110698 [Pubmed: 35844135]
Throughput
- High Throughput
Additional Notes
- Constitutively active mutant of GTPase fused with miniTurboID was stably expressed in HEK293A cells, followed by miniTurboID experiment and TMT-based mass spectrometry. FDR threshold of 0.05
Curated By
- BioGRID