An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.

Li FL, Wu Z, Gao YQ, Bowling FZ, Franklin JM, Hu C, Suhandynata RT, Frohman MA, Airola MV, Zhou H, Guan KL

The Arf GTPase family is involved in a wide range of cellular regulation including membrane trafficking and organelle-structure assembly. Here, we have generated a proximity interaction network for the Arf family using the miniTurboID approach combined with TMT-based quantitative mass spectrometry. Our interactome confirmed known interactions and identified many novel interactors that provide leads for defining Arf pathway cell biological ... [more]

EMBO J Jul. 17, 2022; ();e110698 [Pubmed: 35844135]

Throughput

High Throughput

Additional Notes

Constitutively active mutant of GTPase fused with miniTurboID was stably expressed in HEK293A cells, followed by miniTurboID experiment and TMT-based mass spectrometry. FDR threshold of 0.05

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ARL8B RUFY3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Rawat S (2023)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ARL8B

Gene Ontology Biological Process

Gene Ontology Molecular Function

GDP binding [IDA]GTP binding [IDA]GTPase activity [NAS]alpha-tubulin binding [IDA]beta-tubulin binding [IDA]

Gene Ontology Cellular Component

RUFY3