Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A Combinatorial CRISPR-Cas9 Screen Identifies Ifenprodil as an Adjunct to Sorafenib for Liver Cancer Treatment.

Xu F, Tong M, Tong CSW, Chan BKC, Chu HY, Wong TL, Fong JHC, Cheung MSH, Mak KH, Pardeshi L, Huang Y, Wong KH, Choi GCG, Ma S, Wong ASL

Systematic testing of existing drugs and their combinations is an attractive strategy to exploit approved drugs for repurposing and identifying the best actionable treatment options. To expedite the search among many possible drug combinations, we designed a combinatorial CRISPR-Cas9 screen to inhibit druggable targets. Coblockade of the N-methyl-d-aspartate receptor (NMDAR) with targets of first-line kinase inhibitors reduced hepatocellular carcinoma (HCC) ... [more]

Cancer Res Dec. 15, 2020; 81(24);6219-6232 [Pubmed: 34666996]

Throughput

Low Throughput

Ontology Terms

phenotype: growth abnormality (HP:0001507) [viability (PATO:0000169)]

Additional Notes

CRISPR GI screen
Cell Line: MHCC97L HCC
Experimental Setup: Timecourse
GIST: A-phenotypic negative genetic interaction
Library: Targeted CRISPRn library
Significance Threshold: mean log2 ratios of <-0.51 at P<0.05

Curated By

BioGRID

Interaction Summary

PTK7

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

GRIN3B