BAIT

YGIT

Y75_p2947
DNA-binding transcriptional regulator
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/W3110)
PREY

ROB

Y75_p4280
DNA-binding transcriptional activator
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/W3110)

Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Auxotrophic and prototrophic conditional genetic networks reveal the rewiring of transcription factors in Escherichia coli.

Gagarinova A, Hosseinnia A, Rahmatbakhsh M, Istace Z, Phanse S, Moutaoufik MT, Zilocchi M, Zhang Q, Aoki H, Jessulat M, Kim S, Aly KA, Babu M

Bacterial transcription factors (TFs) are widely studied in Escherichia coli. Yet it remains unclear how individual genes in the underlying pathways of TF machinery operate together during environmental challenge. Here, we address this by applying an unbiased, quantitative synthetic genetic interaction (GI) approach to measure pairwise GIs among all TF genes in E. coli under auxotrophic (rich medium) and prototrophic ... [more]

Nat Commun Jul. 14, 2022; 13(1);4085 [Pubmed: 35835781]

Quantitative Score

  • 2.475328998 [GP score]

Throughput

  • High Throughput

Additional Notes

  • Genetic interactions were considered significant if they had a GP score >= 1.26653954582072 for positive interactions (alleviating interactions) and GP score <= -0.931266889646032 for negative interactions (aggravating interactions).
  • Rich medium

Curated By

  • BioGRID