BAIT

CDC20

PAC5, ubiquitin-protein transferase activating protein CDC20, L000000259, YGL116W
Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

KIP1

CIN9, L000000907, YBL063W
Kinesin-related motor protein; required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Slk19-dependent mid-anaphase pause in kinesin-5-mutated cells.

Movshovich N, Fridman V, Gerson-Gurwitz A, Shumacher I, Gertsberg I, Fich A, Hoyt MA, Katz B, Gheber L

We examined spindle elongation in anaphase in Saccharomyces cerevisiae cells mutated for the kinesin-5 motor proteins Cin8 and Kip1. Cells were deleted for KIP1 and/or expressed one of two motor-domain Cin8 mutants (Cin8-F467A or Cin8-R196K, which differ in their ability to bind microtubules in vitro, with Cin8-F467A having the weakest ability). We found that, in kinesin-5-mutated cells, predominantly in kip1 ... [more]

J. Cell. Sci. Aug. 01, 2008; 121(0);2529-39 [Pubmed: 18628309]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: cell cycle progression in late anaphase (APO:0000263)

Additional Notes

  • overexpression of CDC20 eliminates the anaphase pause seen in KIP1/CIN8 double mutants

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KIP1 CDC20
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
520903

Curated By

  • BioGRID