BAIT
PRMT5
HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs
protein arginine methyltransferase 5
GO Process (13)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- cell proliferation [TAS]
- circadian regulation of gene expression [ISS]
- endothelial cell activation [IMP]
- gene expression [TAS]
- histone H4-R3 methylation [ISS, NAS]
- ncRNA metabolic process [TAS]
- peptidyl-arginine N-methylation [IDA]
- peptidyl-arginine methylation [IMP]
- peptidyl-arginine methylation, to symmetrical-dimethyl arginine [IMP]
- regulation of mitosis [TAS]
- regulation of transcription, DNA-templated [IBA]
- spliceosomal snRNP assembly [IMP, TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
GTF2F1
BTF4, RAP74, TF2F1, TFIIF
general transcription factor IIF, polypeptide 1, 74kDa
GO Process (11)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
- 7-methylguanosine mRNA capping [TAS]
- RNA splicing [TAS]
- gene expression [TAS]
- mRNA splicing, via spliceosome [TAS]
- positive regulation of catalytic activity [IDA]
- positive regulation of viral transcription [TAS]
- response to virus [IEP]
- transcription elongation from RNA polymerase II promoter [TAS]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.687)
- MCF7 cell line (score 0.721)
- MDA231 cell line (score 0.72)
Curated By
- BioGRID