BAIT

EPB41L2

4.1-G, 4.1G, RP3-324N14.1
erythrocyte membrane protein band 4.1-like 2
GO Process (0)
GO Function (0)
GO Component (8)
Homo sapiens
PREY

PRPF40A

FBP-11, FBP11, FLAF1, FNBP3, HIP-10, HIP10, HYPA, NY-REN-6, Prp40, HSPC225
PRP40 pre-mRNA processing factor 40 homolog A (S. cerevisiae)
GO Process (4)
GO Function (2)
GO Component (4)
Homo sapiens

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.

Havugimana PC, Goel RK, Phanse S, Youssef A, Padhorny D, Kotelnikov S, Kozakov D, Emili A

Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]

Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]

Throughput

  • High Throughput

Additional Notes

  • High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
  • MCF10A cell line (score 0.778)
  • MCF7 cell line (score 0.761)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PRPF40A EPB41L2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High14.6675BioGRID
2949096

Curated By

  • BioGRID