BAIT

SYNCRIP

GRY-RBP, GRYRBP, HNRNPQ, HNRPQ1, NSAP1, PP68, hnRNP-Q, RP1-3J17.2
synaptotagmin binding, cytoplasmic RNA interacting protein
Autism spectrum disorder (ASD) Project
Homo sapiens
PREY

ILF2

NF45, PRO3063, RP11-354A16.1
interleukin enhancer binding factor 2
GO Process (2)
GO Function (4)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.

Havugimana PC, Goel RK, Phanse S, Youssef A, Padhorny D, Kotelnikov S, Kozakov D, Emili A

Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]

Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]

Throughput

  • High Throughput

Additional Notes

  • High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
  • MCF10A cell line (score 0.679)
  • MCF7 cell line (score 0.654)
  • MDA231 cell line (score 0.696)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ILF2 SYNCRIP
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9533BioGRID
3153915
SYNCRIP ILF2
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.775BioGRID
744835

Curated By

  • BioGRID