Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.

Havugimana PC, Goel RK, Phanse S, Youssef A, Padhorny D, Kotelnikov S, Kozakov D, Emili A

Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]

Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]

Throughput

High Throughput

Additional Notes

High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
MCF10A cell line (score 0.718)
MCF7 cell line (score 0.673)
MDA231 cell line (score 0.696)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SNX4 SNX2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3371302
SNX2 SNX4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Haft CR (1998)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SNX2

Gene Ontology Biological Process

Gene Ontology Molecular Function

epidermal growth factor receptor binding [IDA]insulin receptor binding [IDA]leptin receptor binding [IDA]phosphatidylinositol binding [IBA]protein binding [IPI]transferrin receptor binding [IDA]

Gene Ontology Cellular Component

SNX4

Gene Ontology Biological Process

Gene Ontology Molecular Function

epidermal growth factor receptor binding [IDA]insulin receptor binding [IDA]leptin receptor binding [IDA]phosphatidylinositol binding [IMP]protein binding [IPI]transferrin receptor binding [IDA]