ROCK2
Gene Ontology Biological Process
- axon guidance [TAS]
- centrosome duplication [IMP]
- cytokinesis [NAS]
- negative regulation of angiogenesis [IMP]
- protein phosphorylation [IDA]
- regulation of actin cytoskeleton organization [TAS]
- regulation of cell adhesion [TAS]
- regulation of cell motility [TAS]
- regulation of circadian rhythm [ISS]
- regulation of establishment of cell polarity [TAS]
- regulation of focal adhesion assembly [TAS]
- regulation of keratinocyte differentiation [IMP]
- regulation of stress fiber assembly [TAS]
- smooth muscle contraction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ROCK1
Gene Ontology Biological Process
- Rho protein signal transduction [TAS]
- apoptotic process [TAS]
- axon guidance [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- leukocyte cell-cell adhesion [IDA]
- leukocyte migration [IDA]
- leukocyte tethering or rolling [IDA]
- membrane to membrane docking [IDA]
- myoblast migration [ISS]
- negative regulation of angiogenesis [IMP]
- positive regulation of focal adhesion assembly [ISS]
- regulation of actin cytoskeleton organization [TAS]
- regulation of cell adhesion [TAS]
- regulation of cell motility [TAS]
- regulation of establishment of cell polarity [TAS]
- regulation of focal adhesion assembly [TAS]
- regulation of keratinocyte differentiation [IMP]
- regulation of stress fiber assembly [TAS]
- signal transduction [TAS]
- smooth muscle contraction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.83)
- MCF7 cell line (score 0.922)
- MDA231 cell line (score 0.927)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ROCK2 ROCK1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2219325 | |
| ROCK2 ROCK1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3074199 | |
| ROCK1 ROCK2 | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | 3682941 |
Curated By
- BioGRID