BAIT
ASS1
ASS, CTLN1, RP11-618A20.2
argininosuccinate synthase 1
GO Process (10)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- arginine biosynthetic process [IBA]
- argininosuccinate metabolic process [IMP]
- aspartate metabolic process [IMP]
- cellular nitrogen compound metabolic process [TAS]
- cellular response to laminar fluid shear stress [IMP]
- citrulline metabolic process [IMP]
- negative regulation of leukocyte cell-cell adhesion [IMP]
- positive regulation of nitric oxide biosynthetic process [IMP]
- small molecule metabolic process [TAS]
- urea cycle [IMP, TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
ENO1
ENO1L1, MPB1, NNE, PPH
enolase 1, (alpha)
GO Process (9)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- gluconeogenesis [TAS]
- glucose metabolic process [TAS]
- glycolytic process [TAS]
- negative regulation of cell growth [IDA]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [IDA]
- response to virus [IEP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF7 cell line (score 0.714)
Curated By
- BioGRID