BAIT
HSPA8
HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71
heat shock 70kDa protein 8
GO Process (14)
GO Function (11)
GO Component (13)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- RNA metabolic process [TAS]
- axon guidance [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- membrane organization [TAS]
- negative regulation of fibril organization [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- neurotransmitter secretion [TAS]
- post-Golgi vesicle-mediated transport [TAS]
- protein folding [NAS]
- protein refolding [IDA]
- response to unfolded protein [NAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function- ATP binding [IDA]
- ATPase activity [IDA]
- ATPase activity, coupled [NAS]
- G-protein coupled receptor binding [IPI]
- MHC class II protein complex binding [IDA]
- enzyme binding [IPI]
- heat shock protein binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [IDA]
- ATP binding [IDA]
- ATPase activity [IDA]
- ATPase activity, coupled [NAS]
- G-protein coupled receptor binding [IPI]
- MHC class II protein complex binding [IDA]
- enzyme binding [IPI]
- heat shock protein binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [IDA]
Gene Ontology Cellular Component
- Prp19 complex [IDA]
- blood microparticle [IDA]
- clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane [TAS]
- cytosol [IDA, TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- intracellular [NAS]
- membrane [IDA]
- nucleus [IDA]
- plasma membrane [TAS]
- ribonucleoprotein complex [IDA]
- ubiquitin ligase complex [IDA]
Homo sapiens
PREY
HDAC2
HD2, RPD3, YAF1
histone deacetylase 2
GO Process (29)
GO Function (13)
GO Component (8)
Gene Ontology Biological Process
- ATP-dependent chromatin remodeling [IDA]
- blood coagulation [TAS]
- chromatin remodeling [IC]
- circadian regulation of gene expression [ISS]
- dendrite development [ISS]
- embryonic digit morphogenesis [ISS]
- epidermal cell differentiation [ISS]
- eyelid development in camera-type eye [ISS]
- fungiform papilla formation [ISS]
- hair follicle placode formation [ISS]
- histone H3 deacetylation [ISS]
- histone H4 deacetylation [ISS]
- histone deacetylation [IMP]
- maintenance of chromatin silencing [IMP]
- negative regulation of MHC class II biosynthetic process [IC]
- negative regulation of apoptotic process [ISS]
- negative regulation of cell cycle [TAS]
- negative regulation of neuron projection development [ISS]
- negative regulation of sequence-specific DNA binding transcription factor activity [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [IC, IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- odontogenesis of dentin-containing tooth [ISS]
- positive regulation of cell proliferation [IMP]
- positive regulation of collagen biosynthetic process [IC]
- positive regulation of proteolysis [IMP]
- positive regulation of receptor biosynthetic process [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription, DNA-templated [IC]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II repressing transcription factor binding [IPI]
- chromatin binding [ISS]
- deacetylase activity [ISS]
- enzyme binding [IPI]
- histone deacetylase activity [IDA]
- nucleosomal DNA binding [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein deacetylase activity [IMP]
- sequence-specific DNA binding [IDA]
- transcription factor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II repressing transcription factor binding [IPI]
- chromatin binding [ISS]
- deacetylase activity [ISS]
- enzyme binding [IPI]
- histone deacetylase activity [IDA]
- nucleosomal DNA binding [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein deacetylase activity [IMP]
- sequence-specific DNA binding [IDA]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.78)
- MCF7 cell line (score 0.804)
- MDA231 cell line (score 0.804)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HDAC2 HSPA8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID