BAIT

PRP45

FUN20, mRNA splicing protein PRP45, L000000638, YAL032C
Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
Saccharomyces cerevisiae (S288c)
PREY

NTC20

L000004267, YBR188C
Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates.

Gahura O, Abrhamova K, Skruzny M, Valentova A, Munzarova V, Folk P, Puta F

Human transcription co-regulator SNW1/SKIP is implicated in the regulation of both transcription elongation and alternative splicing. Prp45, the SNW/SKIP ortholog in yeast, is assumed to be essential for pre-mRNA processing. Here, we characterize prp45(1-169), a temperature sensitive allele of PRP45, which at permissive temperature elicits cell division defects and hypersensitivity to microtubule inhibitors. Using a synthetic lethality screen, we found ... [more]

J. Cell. Biochem. Jan. 01, 2009; 106(1);139-51 [Pubmed: 19016306]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)
  • phenotype: uv resistance (APO:0000085)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PRP45 NTC20
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High7BioGRID
3603381
PRP45 NTC20
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.753BioGRID
1957825

Curated By

  • BioGRID