DDX5
Gene Ontology Biological Process
- cell growth [NAS]
- intrinsic apoptotic signaling pathway by p53 class mediator [IMP]
- mRNA splicing, via spliceosome [IC]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of DNA damage response, signal transduction by p53 class mediator [IMP]
- positive regulation of intracellular estrogen receptor signaling pathway [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of alternative mRNA splicing, via spliceosome [IDA]
- regulation of androgen receptor signaling pathway [IMP]
- regulation of osteoblast differentiation [ISS]
- regulation of skeletal muscle cell differentiation [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HNRNPK
Gene Ontology Biological Process
- RNA processing [TAS]
- RNA splicing [TAS]
- gene expression [TAS]
- mRNA splicing, via spliceosome [IC, TAS]
- positive regulation of low-density lipoprotein particle receptor biosynthetic process [IMP]
- positive regulation of receptor-mediated endocytosis [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of low-density lipoprotein particle clearance [IMP]
- signal transduction [TAS]
Gene Ontology Molecular Function- RNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- single-stranded DNA binding [TAS]
- RNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- single-stranded DNA binding [TAS]
Gene Ontology Cellular Component
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.864)
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
DDX5 HNRNPK | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
HNRNPK DDX5 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID