VCL
Gene Ontology Biological Process
- adherens junction assembly [IMP]
- apical junction assembly [IMP]
- blood coagulation [TAS]
- cell adhesion [TAS]
- cell-matrix adhesion [TAS]
- cellular component movement [TAS]
- epithelial cell-cell adhesion [IMP]
- lamellipodium assembly [ISS]
- morphogenesis of an epithelium [IMP]
- muscle contraction [TAS]
- negative regulation of cell migration [TAS]
- platelet activation [TAS]
- platelet aggregation [IMP]
- platelet degranulation [TAS]
- protein localization to cell surface [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
BCAR1
Gene Ontology Biological Process
- B cell receptor signaling pathway [IDA]
- G-protein coupled receptor signaling pathway [ISS, NAS]
- T cell receptor signaling pathway [NAS]
- actin filament organization [IDA]
- antigen receptor-mediated signaling pathway [IDA]
- blood coagulation [TAS]
- cell chemotaxis [IMP]
- cell division [NAS]
- cell migration [IDA]
- cell proliferation [TAS]
- cellular response to hepatocyte growth factor stimulus [IMP]
- epidermal growth factor receptor signaling pathway [ISS]
- hepatocyte growth factor receptor signaling pathway [IMP]
- insulin receptor signaling pathway [ISS]
- integrin-mediated signaling pathway [IDA]
- neurotrophin TRK receptor signaling pathway [ISS]
- platelet activation [TAS]
- platelet-derived growth factor receptor signaling pathway [ISS]
- positive regulation of cell migration [IDA]
- positive regulation of endothelial cell migration [IDA]
- regulation of apoptotic process [TAS]
- regulation of cell growth [TAS]
- vascular endothelial growth factor receptor signaling pathway [IMP]
Gene Ontology Molecular Function
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.666)
- MCF7 cell line (score 0.647)
- MDA231 cell line (score 0.687)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BCAR1 VCL | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| VCL BCAR1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 25 | BioGRID | 3010473 | |
| VCL BCAR1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2695856 |
Curated By
- BioGRID