BAIT

PDI1

MFP1, TRG1, protein disulfide isomerase PDI1, L000001360, YCL043C
Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
GO Process (1)
GO Function (4)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SPF1

COD1, PER9, PIO1, ion-transporting P-type ATPase SPF1, L000003454, YEL031W
P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The unfolded protein response is necessary but not sufficient to compensate for defects in disulfide isomerization.

Kim JH, Zhao Y, Pan X, He X, Gilbert HF

Pdi1p (protein-disulfide isomerase) is a folding assistant of the endoplasmic reticulum (ER) that catalyzes disulfide formation and the isomerization of incorrect disulfides. Its disulfide forming activity is its essential function in Saccharomyces cerevisiae. A truncation mutant (Pdi1a') that is competent in disulfide formation but deficient in catalyzing isomerization has only a small effect on growth, although the maturation of isomerase-requiring ... [more]

J. Biol. Chem. Apr. 17, 2009; 284(16);10400-8 [Pubmed: 19233841]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: heat sensitivity (APO:0000147)
  • phenotype: inviable (APO:0000112)

Additional Notes

  • diploid synthetic lethality array method (dSLAM)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SPF1 PDI1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-BioGRID
211754
PDI1 SPF1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.8738BioGRID
209917

Curated By

  • BioGRID