PDI1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SEC66
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
The unfolded protein response is necessary but not sufficient to compensate for defects in disulfide isomerization.
Pdi1p (protein-disulfide isomerase) is a folding assistant of the endoplasmic reticulum (ER) that catalyzes disulfide formation and the isomerization of incorrect disulfides. Its disulfide forming activity is its essential function in Saccharomyces cerevisiae. A truncation mutant (Pdi1a') that is competent in disulfide formation but deficient in catalyzing isomerization has only a small effect on growth, although the maturation of isomerase-requiring ... [more]
Throughput
- High Throughput|Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
- phenotype: heat sensitivity (APO:0000147)
Additional Notes
- Low throughput verification of the synthetic lethal phenotype of a subset of genes identified by dSLAM. The experiment involved the temperature-sensitive PDI1 gene (tsPDIa') and was carried out at the semipermissive temperature of 30 degrees Celsius.
- diploid synthetic lethality array method (dSLAM)
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
PDI1 SEC66 | Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores. | High | 2.8494 | BioGRID | 209924 | |
SEC66 PDI1 | Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores. | High | - | BioGRID | 211494 |
Curated By
- BioGRID