BAIT

CDC21

CRT9, TMP1, thymidylate synthase, YOR29-25, L000000260, YOR074C
Thymidylate synthase; required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

UNG1

L000002428, YML021C
Uracil-DNA glycosylase; required for repair of uracil in DNA formed by spontaneous cytosine deamination; efficiently excises uracil from single-stranded DNA in vivo; not required for strand-specific mismatch repair; cell-cycle regulated, expressed in late G1; localizes to mitochondria and nucleus
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Ung1p-mediated uracil-base excision repair in mitochondria is responsible for the petite formation in thymidylate deficient yeast.

Chien CY, Chou CK, Su JY

The budding yeast CDC21 gene, which encodes thymidylate synthase, is crucial in the thymidylate biosynthetic pathway. Early studies revealed that high frequency of petites were formed in heat-sensitive cdc21 mutants grown at the permissive temperature. However, the molecular mechanism involved in such petite formation is largely unknown. Here we used a yeast cdc21-1 mutant to demonstrate that the mutant cells ... [more]

FEBS Lett. May. 06, 2009; 583(9);1499-504 [Pubmed: 19362086]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: petite (APO:0000153)
  • phenotype: viability (APO:0000111)

Additional Notes

  • deletion of UNG1 increases growth rate in a CDC21 mutant and decreases the rate of petite colonies

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UNG1 CDC21
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2465BioGRID
2059863

Curated By

  • BioGRID