Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.

Havugimana PC, Goel RK, Phanse S, Youssef A, Padhorny D, Kotelnikov S, Kozakov D, Emili A

Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]

Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]

Throughput

High Throughput

Additional Notes

High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
MDA231 cell line (score 0.691)

Curated By

BioGRID

Interaction Summary

PRDX3

Gene Ontology Biological Process

Gene Ontology Molecular Function

alkyl hydroperoxide reductase activity [NAS]cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IMP]kinase binding [IPI]protein C-terminus binding [IPI]protein binding [IPI]protein kinase binding [IPI]thioredoxin peroxidase activity [IDA]

Gene Ontology Cellular Component

CAP1