BAIT
KIF5B
HEL-S-61, KINH, KNS, KNS1, UKHC
kinesin family member 5B
GO Process (10)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- axon guidance [IBA]
- cellular protein metabolic process [TAS]
- cytoskeleton-dependent intracellular transport [IBA]
- microtubule-based movement [IBA]
- positive regulation of establishment of protein localization to plasma membrane [IDA]
- positive regulation of potassium ion transport [IDA]
- regulation of membrane potential [IDA]
- stress granule disassembly [ISS]
- vesicle transport along microtubule [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PFN2
D3S1319E, PFL
profilin 2
GO Process (8)
GO Function (4)
GO Component (1)
Gene Ontology Biological Process
- negative regulation of actin filament polymerization [IDA]
- negative regulation of epithelial cell migration [IMP]
- negative regulation of ruffle assembly [IMP]
- positive regulation of ATPase activity [IDA]
- positive regulation of actin filament bundle assembly [IMP]
- positive regulation of actin filament polymerization [IGI]
- positive regulation of peptidyl-serine phosphorylation [IMP]
- positive regulation of stress fiber assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF7 cell line (score 0.69)
Curated By
- BioGRID